Yeast culture for maintaining and propagating various strains, such as Saccharomyces cerevisiae (S. cerevisiae) and Pichia pastoris (P. pastoris), requires specific formulations of complex media for use in cloning and protein expression.We offer a selection of yeast growth media for your specific application in both powder and ready-to-use liquid formats for convenience and ease of use.
Supplementary Materials for Jun 05, 2020 Unanticipated Heterogeneity in Growth Rate and Virulence The disruption of a specific gene in Candida albicans is commonly used to determine the function of the gene product. We disrupted AAF1 , a gene of C. albicans that causes Saccharomyces cerevisiae to flocculate and adhere to endothelial cells. We then characterized multiple heterozygous and homozygous mutants. These null mutants adhered to endothelial cells to the same extent as did the parent Bacto™ Peptone - US Bacto Peptone is an animal origin (AO) enzymatic digest of bovine and porcine animal proteins. The nutritive value of Bacto Peptone is largely dependent on a combination of a negligible quantity of proteases, high concentration of complex constituents, and a unique amino acid content that makes this peptone a strong nitrogen source. THE J B C © 2002 by The American Society for Biochemistry
Engineered Pichia pastoris production of fusaruside, a
Buffering the pH of the culture medium does not extend Oct 15, 2013 YPD Broth for microbiology | Sigma-Aldrich
A nutritious general liquid growth medium for the propagation of yeast. Components Ingredients (g/L) Bacteriological peptone, 20 Yeast extract, 10 Glucose, 20 Quantity 50 g per liter Reconstitution Preparation instructions 1. Suspend 50 g in 1 L of distilled water. 2. Autoclave for 15 minutes at 121 °C.
Yeast cells were grown in rich (YPD) medium containing 10 g L −1 yeast extract, 20 g L −1 Bacto peptone and different glucose concentrations (as indicated; 20, 10, 5 or 2.5 g L −1). YPKG contained 10 g L −1 Bacto yeast extract, 20 g L −1 Bacto peptone, 10 g L −1 potassium acetate and 5 g L −1 glucose. Cell growth was followed by